DNA

Part:BBa_K1039011:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-10)


PhiC31 Invertible Promoter Switch (Promoter J23118) – RL State


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that there is an NheI site on the prefix side of the switch before the attR site. This was placed there as part of an early design of our switch, but does not contribute to functionality.



Source

This sequence was synthesized from BioBasic Inc.

References